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Tuesday, March 22, 2005
Ordinary Day
I woke up this morning expecting to get into the lab a little before 10 to watch a few procedures in the PC2 room (basically a sterile room) and get acquainted with what I would need to be doing for my cell cultures. I wandered down there at about 10 to find Christina there working with her cells and got to watch her for a bit, and then expected to help out with the defrosting of a cell line that I might be using. Instead, a head poked into the PC2 room and informed Chris that a sheep had been sacrificed shortly before after having a severe reaction to the anesthetic it had been given. She finished up her work quick and we headed over towards the surgery room where the sheep was already on ice. (Feel free to skip the rest of this paragraph as it is slightly gory). We suited up in surgical overalls, with eye protection and gloves and, using a crane, moved the sheep out of the freezer onto a dolly (no pun intended) and rolled it into the surgical anteroom. Then, between myself, Chris, and one of the other staff members there we removed the 2 rear legs so Chris could get at the chondrocytes in the knee capsule. It was really interesting to watch the layers of skin, fat and muscle get cut through as we made the way to the bone, which we needed a hacksaw to get through. The legs were then double bagged and into the cold room they went, while the rest of the sheep went back in the freezer. After that little adventure we met up with Jennifer who was defrosting the cells, and decided that it would be done at 2pm. I popped home for a quick lunch and then we went to work on defrosting the cells, and seeding them after their resuspension in media. Then I got to help make up a batch of new media, and stand with a pH meter adding drop after drop of HCl till it was sitting nicely at a pH of 7.12. The last step was filtering it, which took a bit of time, but was eventually completed. Finally I ran off to class only a little late, made it through the lecture and back home through the pouring rain. Now its just working on my assignments and getting ready for the break which is rapidly approaching.
Comments:
Sounds like quite a day. Just as we thought, you're going to be doing great thimgs for humanity.
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